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pt2 huc dendra2 plasmid  (Addgene inc)


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    Structured Review

    Addgene inc pt2 huc dendra2 plasmid
    Pt2 Huc Dendra2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pt2+huc+dendra2+plasmid/pMT-HuC%3ADendra2+(Plasmid+%2380904)/pmc11306173-51-8-14
    Average 91 stars, based on 4 article reviews
    pt2 huc dendra2 plasmid - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A decrease in Fkbp52 alters autophagosome maturation and A152T-tau clearance in vivo
    Article Snippet: To obtain the pT2-HuC:Lamp1-mEGFP plasmid, a lamp1-mEGFP cassette flanked by SpeI and MluI sites was PCR-amplified with 5′-GAATTCACTAGTATGGCGGCCCCCGGCAG-3′ forward and 5′-TGTATCACGCGTTTACTTGTACAGCTCGTCC-3′ reverse primers using Addgene plasmid #34831 (gift from Esteban Dell’Angelica) ( ) as template. .. The resulting DNA fragment was subcloned into the pT2-HuC:Dendra2 plasmid (gift from Periklis Pantazis, Addgene #80904) ( ) previously modified to introduce multiple cloning sites 3′ to the Dendra2 sequence, replacing Dendra2. ..

    Modification:

    Article Title: A decrease in Fkbp52 alters autophagosome maturation and A152T-tau clearance in vivo
    Article Snippet: To obtain the pT2-HuC:Lamp1-mEGFP plasmid, a lamp1-mEGFP cassette flanked by SpeI and MluI sites was PCR-amplified with 5′-GAATTCACTAGTATGGCGGCCCCCGGCAG-3′ forward and 5′-TGTATCACGCGTTTACTTGTACAGCTCGTCC-3′ reverse primers using Addgene plasmid #34831 (gift from Esteban Dell’Angelica) ( ) as template. .. The resulting DNA fragment was subcloned into the pT2-HuC:Dendra2 plasmid (gift from Periklis Pantazis, Addgene #80904) ( ) previously modified to introduce multiple cloning sites 3′ to the Dendra2 sequence, replacing Dendra2. ..

    Introduce:

    Article Title: A decrease in Fkbp52 alters autophagosome maturation and A152T-tau clearance in vivo
    Article Snippet: To obtain the pT2-HuC:Lamp1-mEGFP plasmid, a lamp1-mEGFP cassette flanked by SpeI and MluI sites was PCR-amplified with 5′-GAATTCACTAGTATGGCGGCCCCCGGCAG-3′ forward and 5′-TGTATCACGCGTTTACTTGTACAGCTCGTCC-3′ reverse primers using Addgene plasmid #34831 (gift from Esteban Dell’Angelica) ( ) as template. .. The resulting DNA fragment was subcloned into the pT2-HuC:Dendra2 plasmid (gift from Periklis Pantazis, Addgene #80904) ( ) previously modified to introduce multiple cloning sites 3′ to the Dendra2 sequence, replacing Dendra2. ..

    Cloning:

    Article Title: A decrease in Fkbp52 alters autophagosome maturation and A152T-tau clearance in vivo
    Article Snippet: To obtain the pT2-HuC:Lamp1-mEGFP plasmid, a lamp1-mEGFP cassette flanked by SpeI and MluI sites was PCR-amplified with 5′-GAATTCACTAGTATGGCGGCCCCCGGCAG-3′ forward and 5′-TGTATCACGCGTTTACTTGTACAGCTCGTCC-3′ reverse primers using Addgene plasmid #34831 (gift from Esteban Dell’Angelica) ( ) as template. .. The resulting DNA fragment was subcloned into the pT2-HuC:Dendra2 plasmid (gift from Periklis Pantazis, Addgene #80904) ( ) previously modified to introduce multiple cloning sites 3′ to the Dendra2 sequence, replacing Dendra2. ..

    Sequencing:

    Article Title: A decrease in Fkbp52 alters autophagosome maturation and A152T-tau clearance in vivo
    Article Snippet: To obtain the pT2-HuC:Lamp1-mEGFP plasmid, a lamp1-mEGFP cassette flanked by SpeI and MluI sites was PCR-amplified with 5′-GAATTCACTAGTATGGCGGCCCCCGGCAG-3′ forward and 5′-TGTATCACGCGTTTACTTGTACAGCTCGTCC-3′ reverse primers using Addgene plasmid #34831 (gift from Esteban Dell’Angelica) ( ) as template. .. The resulting DNA fragment was subcloned into the pT2-HuC:Dendra2 plasmid (gift from Periklis Pantazis, Addgene #80904) ( ) previously modified to introduce multiple cloning sites 3′ to the Dendra2 sequence, replacing Dendra2. ..



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    91
    Addgene inc pt2 huc dendra2 plasmid
    Pt2 Huc Dendra2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pt2+huc+dendra2+plasmid/pMT-HuC%3ADendra2+(Plasmid+%2380904)/pmc11306173-51-8-14
    Average 91 stars, based on 1 article reviews
    pt2 huc dendra2 plasmid - by Bioz Stars, 2026-09
    91/100 stars
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